@techreport{oai:ipsj.ixsq.nii.ac.jp:00081531,
 author = {山田, 恵 and 清水（稲継）, 理恵 and 清水, 健太郎 and 瀬々, 潤 and Megumi, Yamada and Rie, Shimizu(Inatsugi) and Kentaro, Shimizu and Jun, Sese},
 issue = {1},
 month = {Mar},
 note = {本研究では，異なる種のゲノムを併せ持つ種である異質倍数体の遺伝子発現量をRNA-seqにより定量化した．しかし，異質倍数体の親となる種は近縁の種で構成されることが多いため，通常のRNA-seqでは親種同士のホモログ遺伝子の配列が非常に近く，いずれの遺伝子が発現したかを見分けることが困難である．本研究では，近縁種からの変異のパターンに着目し，最尤推定法を用いることで，親種を分けた発現量の定量化を可能とした．, In this research, We tackled a measurement of the expression levels of genes in alloploidies. For the measurement, we used an RNA-seq. However, high sequence similarity between genes originating from different parental species prevented us to apply the method to the problem directly. To overcome the difficulty, our method used genome of model species, which is closely related to the target alloploid, and aligned RNA-seq sequences to the genome. Based on the mutation distributions of the alignment result, our method determined the originated species of each read using maximum likelihood estimation method. By counting the number of the sequences of each parental species on each gene, our method successfully quantified expression levels of genes in allopolyploid species.},
 title = {異質倍数体の遺伝子発現量の定量化},
 year = {2012}
}