@techreport{oai:ipsj.ixsq.nii.ac.jp:00238565, author = {Kohei, Bamba and Ryo, Harada and Yuji, Inagaki and Kohei, Bamba and Ryo, Harada and Yuji, Inagaki}, issue = {1}, month = {Aug}, note = {Both inference of a tree diagram and evaluating the credibility of the inferred tree are indispensable in molecular phylogeny. To evaluate the credibility of bipartitions in a phylogenetic tree in the maximum-likelihood framework, bootstrap, UFBoot2, and SH-aLRT are available. In this study, we propose a new software ”AUTOEB,” which evaluates bipartitions in a given tree employing an approximately unbiased (AU) test. For each bipartition, the software generates two alternative trees from a given tree by disrupting the bipartition of interest with the minimum changes in tree topology and compares them by the AU test. In the case of either or both alternative trees failing to be rejected, the software calls the particular bipartition ”unresolved” and otherwise “unresolved.” We here compare the credibility of bipartitions calculated from multiple empirical sequence data by bootstrap, UFBoot2, SH-aLRT, and AUTOEB, and demonstrate that the new method can complement the preexisting ones., Both inference of a tree diagram and evaluating the credibility of the inferred tree are indispensable in molecular phylogeny. To evaluate the credibility of bipartitions in a phylogenetic tree in the maximum-likelihood framework, bootstrap, UFBoot2, and SH-aLRT are available. In this study, we propose a new software ”AUTOEB,” which evaluates bipartitions in a given tree employing an approximately unbiased (AU) test. For each bipartition, the software generates two alternative trees from a given tree by disrupting the bipartition of interest with the minimum changes in tree topology and compares them by the AU test. In the case of either or both alternative trees failing to be rejected, the software calls the particular bipartition ”unresolved” and otherwise “unresolved.” We here compare the credibility of bipartitions calculated from multiple empirical sequence data by bootstrap, UFBoot2, SH-aLRT, and AUTOEB, and demonstrate that the new method can complement the preexisting ones.}, title = {AUTOEB: A software for systematically evaluating bipartitions in a phylogenetic tree employing an approximately unbiased test}, year = {2024} }