Combfit: A Normalization Method for Array CGH Data

• Item type: Trans(1)
• 公開日: 2006-11-15
• タイトル: Combfit: A Normalization Method for Array CGH Data
• タイトル / 言語: en
• タイトル: Combfit: A Normalization Method for Array CGH Data
• 言語: eng
• キーワード / 主題Scheme: Other
• キーワード / 主題: Original Papers
• 資源タイプ / 資源タイプ識別子: http://purl.org/coar/resource_type/c_6501
• 資源タイプ: journal article
• 著者所属: Graduate School of Information Science Nara Institute of Science and Technology
• 著者所属: The 1st Department of Surgery Hokkaido University Graduate School of Medicine
• 著者所属: Division of Biochemistry Chiba Cancer Center Research Institute
• 著者所属: Graduate School of Informa
• 著者所属(英): en
• 著者所属(英): Graduate School of Information Science, Nara Institute of Science and Technology
• 著者所属(英): en
• 著者所属(英): The 1st Department of Surgery, Hokkaido University, Graduate School of Medicine
• 著者所属(英): en
• 著者所属(英): Division of Biochemistry, Chiba Cancer Center Research Institute
• 著者所属(英): en
• 著者所属(英): Graduate School of Information Science, Nara Institute of Science and Technology
• 著者名: Shigeyuki, Oba; Nobumoto, Tomioka; Miki, Ohira; Shin, Ishii
• 著者名(英): Shigeyuki, Oba; Nobumoto, Tomioka; Miki, Ohira; Shin, Ishii
• 論文抄録 / 内容記述タイプ: Other
• 論文抄録 / 内容記述: The recently developed array-based comparative genomic hybridization (array CGH) technique measures DNA copy number aberrations that occur as causes or consequences of cell diseases such as cancers. Conventional array CGH analysis classifies DNA copy number aberrations into three categories: no significant change significant gain and significant loss. However recent improvements in microarray measurement precision enable more quantitative analysis of copy number aberrations. We propose a method called comb fitting that extracts a quantitative interpretation from array CGH data. We also propose modifications that allow us to apply comb fitting to cases featuring heterogeneity of local aberrations in DNA copy numbers. By using comb fitting we can correct the baseline of the fluorescence ratio data measured by array CGH and simultaneously translate them into the amount of changed copy numbers for each small part of the chromosome such as 0 +-1 +-2 ...Comb fitting is applicable even when a considerable amount of contamination by normal cells exists and when heterogeneity in the ploidy number cannot be neglected.
• 論文抄録(英) / 内容記述タイプ: Other
• 論文抄録(英) / 内容記述: The recently developed array-based comparative genomic hybridization (array CGH) technique measures DNA copy number aberrations that occur as causes or consequences of cell diseases such as cancers. Conventional array CGH analysis classifies DNA copy number aberrations into three categories: no significant change, significant gain, and significant loss. However, recent improvements in microarray measurement precision enable more quantitative analysis of copy number aberrations. We propose a method, called comb fitting, that extracts a quantitative interpretation from array CGH data. We also propose modifications that allow us to apply comb fitting to cases featuring heterogeneity of local aberrations in DNA copy numbers. By using comb fitting, we can correct the baseline of the fluorescence ratio data measured by array CGH and simultaneously translate them into the amount of changed copy numbers for each small part of the chromosome, such as 0, +-1, +-2, ...Comb fitting is applicable even when a considerable amount of contamination by normal cells exists and when heterogeneity in the ploidy number cannot be neglected.
• 書誌レコードID / 収録物識別子タイプ: NCID
• 書誌レコードID / 収録物識別子: AA12177013
• 書誌情報: IPSJ Transactions on Bioinformatics （TBIO）; 巻 47, 号 SIG17(TBIO1), p. 73-82, 発行日 2006-11-15
• ISSN / 収録物識別子タイプ: ISSN
• ISSN / 収録物識別子: 1882-6679
• 出版者 / 言語: ja
• 出版者: 情報処理学会