Comprehensive miRNA expression analysis in peripheral blood can diagnose liver disease

• Item type: SIG Technical Reports(1)
• 公開日: 2012-11-29
• タイトル: Comprehensive miRNA expression analysis in peripheral blood can diagnose liver disease
• タイトル / 言語: en
• タイトル: Comprehensive miRNA expression analysis in peripheral blood can diagnose liver disease
• 言語: eng
• 資源タイプ / 資源タイプ識別子: http://purl.org/coar/resource_type/c_18gh
• 資源タイプ: technical report
• 著者所属: Center for Genomic Medicine, Kyoto University Graduate School of Medicine／Presently with Department of Hepatology, Graduate School of Medicine, Osaka City University
• 著者所属: Department of Gastroenterology, Ogaki Municipal Hospital
• 著者所属: Department of Medical Pharmaceutics,Kobe Pharmaceutical University
• 著者所属: Department of Epidemiology, Infectious Disease Control and Prevention, Hiroshima University Graduate School of Biomedical Sciences
• 著者所属: Department of Gastroenterology, Ogaki Municipal Hospital
• 著者所属: Division of Molecular and Cellular Medicine, National Cancer Center Research Institute
• 著者所属: Division of Molecular and Cellular Medicine, National Cancer Center Research Institute
• 著者所属: Division of Molecular and Cellular Medicine, National Cancer Center Research Institute
• 著者所属: Department of Physics, Chuo University
• 著者所属(英): en
• 著者所属(英): Center for Genomic Medicine, Kyoto University Graduate School of Medicine / Presently with Department of Hepatology, Graduate School of Medicine, Osaka City University
• 著者所属(英): en
• 著者所属(英): Department of Gastroenterology, Ogaki Municipal Hospital
• 著者所属(英): en
• 著者所属(英): Department of Medical Pharmaceutics,Kobe Pharmaceutical University
• 著者所属(英): en
• 著者所属(英): Department of Epidemiology, Infectious Disease Control and Prevention, Hiroshima University Graduate School of Biomedical Sciences
• 著者所属(英): en
• 著者所属(英): Department of Gastroenterology, Ogaki Municipal Hospital
• 著者所属(英): en
• 著者所属(英): Division of Molecular and Cellular Medicine, National Cancer Center Research Institute
• 著者所属(英): en
• 著者所属(英): Division of Molecular and Cellular Medicine, National Cancer Center Research Institute
• 著者所属(英): en
• 著者所属(英): Division of Molecular and Cellular Medicine, National Cancer Center Research Institute
• 著者所属(英): en
• 著者所属(英): Department of Physics, Chuo University
• 著者名: Yoshiki, Murakami; Hidenori, Toyoda; Toshihito, Tanahashi; Junko, Tanaka; Takashi, Kumada; Yusuke, Yoshioka; Nobuyoshi, Kosaka; Takahiro, Ochiya; Y-H.Taguchi
• 著者名(英): Yoshiki, Murakami; Hidenori, Toyoda; Toshihito, Tanahashi; Junko, Tanaka; Takashi, Kumada; Yusuke, Yoshioka; Nobuyoshi, Kosaka; Takahiro, Ochiya; Y-H., Taguchi
• 論文抄録 / 内容記述タイプ: Other
• 論文抄録 / 内容記述: Background: miRNAs circulating in the blood in a cell-free form have been acknowledged for their potential as readily accessible disease markers. Presently, histological examination is the golden standard for diagnosing and grading liver disease, therefore non-invasive options are desirable. Here, we investigated if miRNA expression profile in exosome rich fractionated serum could be useful for determining the disease parameters in patients with chronic hepatitis C (CHC). Methodology: Exosome rich fractionated RNA was extracted from the serum of 64 CHC and 24 controls with normal liver (NL). Extracted RNA was subjected to miRNA profiling by microarray and real-time qPCR analysis. The miRNA expression profiles from 4 chronic hepatitis B (CHB) and 12 non alcoholic steatohepatitis (NASH) patients were also established. The resulting miRNA expression was compared to the stage or grade of CHC determined by blood examination and histological inspection. Principal Findings: miRNAs implicated in chronic liver disease and inflammation showed expression profiles that differed from those in NL and varied among the types and grades of liver diseases. Using the expression patterns of twelve miRNAs, we classified CHC, CHB, NASH and NL with 95.25% accuracy. Additionally, we could link miRNA expression pattern with liver fibrosis stage and grade of liver inflammation in CHC. In particular, the miRNA expression pattern for early fibrotic stage differed greatly from that observed in high inflammation grades. Conclusions: We demonstrated that miRNA expression pattern in exosome rich fractionated serum shows a high potential as a biomarker for diagnosing the grade and stage of liver diseases
• 論文抄録(英) / 内容記述タイプ: Other
• 論文抄録(英) / 内容記述: Background: miRNAs circulating in the blood in a cell-free form have been acknowledged for their potential as readily accessible disease markers. Presently, histological examination is the golden standard for diagnosing and grading liver disease, therefore non-invasive options are desirable. Here, we investigated if miRNA expression profile in exosome rich fractionated serum could be useful for determining the disease parameters in patients with chronic hepatitis C (CHC). Methodology: Exosome rich fractionated RNA was extracted from the serum of 64 CHC and 24 controls with normal liver (NL). Extracted RNA was subjected to miRNA profiling by microarray and real-time qPCR analysis. The miRNA expression profiles from 4 chronic hepatitis B (CHB) and 12 non alcoholic steatohepatitis (NASH) patients were also established. The resulting miRNA expression was compared to the stage or grade of CHC determined by blood examination and histological inspection. Principal Findings: miRNAs implicated in chronic liver disease and inflammation showed expression profiles that differed from those in NL and varied among the types and grades of liver diseases. Using the expression patterns of twelve miRNAs, we classified CHC, CHB, NASH and NL with 95.25% accuracy. Additionally, we could link miRNA expression pattern with liver fibrosis stage and grade of liver inflammation in CHC. In particular, the miRNA expression pattern for early fibrotic stage differed greatly from that observed in high inflammation grades. Conclusions: We demonstrated that miRNA expression pattern in exosome rich fractionated serum shows a high potential as a biomarker for diagnosing the grade and stage of liver diseases
• 書誌レコードID / 収録物識別子タイプ: NCID
• 書誌レコードID / 収録物識別子: AA12055912
• 書誌情報: 研究報告バイオ情報学（BIO）; 巻 2012-BIO-32, 号 12, p. 1-6, 発行日 2012-11-29
• Notice: SIG Technical Reports are nonrefereed and hence may later appear in any journals, conferences, symposia, etc.
• 出版者 / 言語: ja
• 出版者: 情報処理学会